Disease Detection in Citrus

Huanglongbing (HLB) disease has arrived in California and is deadly to citrus trees. Trained working canines can detect an infected tree 2-8 months earlier than currently approved detection methods. Canine detection of Citrus Canker and HLB disease in citrus groves and nurseries have demonstrated a very high level of accuracy. We would like to bring trained canines from Florida to California to work with others towards the goal of saving the California Citrus Industry from HLB.

Watch The Video "Save Our Citrus"

"Citron"- Alerting on a HLB Infected Tree

"Citron", pictured above, is one of the first dogs trained to detect HLB in 2007. Now retired, she was owned and trained by J & K Canine Academy/Scentworks.

New Approaches to Detection: Canine Surveillance of High Risk Pathogens

The underpinning for control of exotic diseases such as citrus Huanglongbing (HLB) caused by Candidatus Liberibacter asiaticus (CLas) and Plum Pox Virus (PPV) is early detection/response when incidence is low. Unfortunately, these pathogens can remain asymptomatic and subclinical to PCR detection for months yet can act as inoculum sources. Twenty canines were trained for early detection of HLB and two for PPV. Ten canines were each tested against 1000 trees in replicated randomized field trials with varying HLB-incidence, which resulted in 99.16% overall detection accuracy with very few false negatives or positives. Canines also detected infected trees exclusively from 5-gm feeder root samples. In a time-course experiment, canines detected infections within 2–3 weeks of vector transmission, whereas inoculated trees were not PCR-positive for CLas until at least 3–12 mos. post inoculation. In citrus and prunus field trials, canines trot along the rows with an average interrogation time of ~2–10 trees/s; faster than any other detection method. Canines were also effectively utilized for detection of infected trees in residential areas. This confirms that canines are a very early, accurate and sensitive detection methodology. Canines are able to detect the pathogen in trees with subclinical infection, i.e., before symptom expression and considerably prior to the ability of PCR detection.

ICPP 2018 Abstracts of Concurrent Session Presentations: Phytopathology Supplement July 29-August 3, 2018

T. R. GOTTWALD (1), G. Poole (2), G. Mccollum (2), W. Luo PhD (3), F. Louws (4), (1) USDA-ARS, Ft Pierce, FL, USA; (2) USDA, ARS, Fort

Pierce, FL, USA; (3) USDA ARS & NCSU, Ft Pierce, FL, USA; (4) North Carolina State University, Raleigh, NC, USA

Trained Dogs Set to Sniff Out Deadly Citrus Disease

The Fresno Bee: September 17, 2018

Link to Article

HLB Early Detection Methods Available Now; More Coming Soon

Citrus Industry: October 5, 2018

Link to Article

HLB Detection Dogs Coming to California

Citrus Industry: August 22, 2018

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Interview about HLB and Canker Detection Dogs 

Trained by J & K Canine Academy

AgNeTVideo: Citrus Expo 2013-Scent Worx

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"Juice"- The First Canker Detection Canine

Citrus Canker is a disease caused by the bacterium Xanthomonas axonopodis. Infection causes lesions on the leaves, stems, and fruit of citrus trees, including lime, oranges, and grapefruit. Dogs trained by J & K Canine Academy on Canker were a part of a USDA ARS Research Project in Florida. The research results show the canker dogs were over 99% accurate.  Pepe Peruyero, owner of J & K Canine Academy/Scentworxs in Florida is pictured above with "Juice".

Disease-Detecting Dogs

Citrus Industry: February 2012

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Disease-Detecting Dogs

Belize Ag Report: March-April 2012 (Re-print)

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Research on J & K Canine Academy Dogs Detection of Termites

Journal of Economic Entomology.   96 (4) : 1259-1266 (2003)

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Research on J & K Canine Academy Dogs Detection of Bed Bugs

Journal of Economic Entomology.

101 (4) : 1389 - 1396 (2008)

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Research on J & K Canine Academy Dogs Detecting Sea Turtle Eggs

Marine Turtle Newsletter

152 : 1-4

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AgNeTVideos: J & K Canines Effective at Citrus Canker Detection

October 2012

 Interview About Scentworx Training Dogs For Agriculture on WDSCTV 

2014

Termite Detection Dog Video 

2008

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